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bglH [2019-06-19 12:30:30]
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bglH [2019-06-19 12:30:30]

phospho-beta-glucosidase
locus
BSU39260
pI
4.96
mw
53.12 kDa
protein length
469 aa Sequence Blast
gene length
1410 bp Sequence Blast
function
salicin utilization
product
phospho-beta-glucosidase
essential
no
ec
3.2.1.86
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.18|Utilization of beta-glucosides]

    • Gene

    Coordinates
    4,032,346 4,033,755

    The protein

    Catalyzed reaction/ biological activity

  • 6-phospho-beta-D-glucosyl-(1,4)-D-glucose H2O = D-glucose D-glucose 6-phosphate (according to Swiss-Prot)

    • Protein family

  • glycosyl hydrolase 1 family (according to Swiss-Prot)

    • Structure

  • [PDB|4IPL] (from ''Streptococcus pneumoniae'', 60% identity) [Pubmed|23580646]

    • Expression and Regulation

    Operons

    genes
    [gene|35E6C81BE481C3E57CF47B781580368C3AC15D83|bglP]-[gene|8D98212930CF6613D332BF99452B231CB6426E93|bglH]-[gene|5A4CDE1CFE319E4471A15702ED1632C96270528D|yxiE]
    description
    [Pubmed|7883710]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7559347], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA] binding site overlaps -35 region) [Pubmed|7559347], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|LicT]: anti-termination, via [protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|LicT]-dependent [SW|RNA switch], lack of [protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|LicT]-dependent antitermination in the presence of gucose due to the requirement of [protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|LicT] to be phosphorylated by [protein|771F205F818A755A661B8E1C95365A4F6AEB05C7|HprK], in [regulon|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|LicT regulon]

    • regulation

  • induced by salicin ([protein|search|LicT]) [Pubmed|7883710]

    • additional information

  • An [ncRNA|search|antisense RNA] is predicted for '[protein|search|bglP]' [PubMed|20525796]
    • view in new tab

    Biological materials

    Mutant

  • BKE39260 ([gene|8D98212930CF6613D332BF99452B231CB6426E93|bglH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGATCACCCCATTAG, downstream forward: _UP4_TGACAATTTTTTGAAAACTC
  • BKK39260 ([gene|8D98212930CF6613D332BF99452B231CB6426E93|bglH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGATCACCCCATTAG, downstream forward: _UP4_TGACAATTTTTTGAAAACTC

    • References

  • 14652714,16672620,15139916,7883710,7559347,26772920,23580646